dapi vector laboratories h 1800 cholera toxin subunit b recombinant Search Results


99
Developmental Studies Hybridoma Bank 632496 mab anti bruchpilot dshb nc82 mab anti β galactosidase promega z3781 mab anti calcineurin β subunit
(a) Representative confocal slice images of P1a neuron cell bodies in control vs fru mutant males in different social conditions. Flies were reared singly for 3 days upon eclosion and then paired with indicated partners for an additional 3-day period. (b) NLI of CRTC::GFP signal in P1a neurons of control vs fru mutant males in different conditions. Each dot represents mean NLI across cells per fly. Boxplot; median (horizontal line), first and third quartiles (box), and data within 1.5x IQR (whiskers). Statistics by Kruskal-Wallis test followed by a post hoc Tukey’s HSD test; *** p<0.001, ** p<0.01, * p<0.05, n.s. p>0.05. (c) Confocal images of P1a neurons expressing GCaMP in control and fru mutant males. Dotted area represents approximate plane for calcium imaging. <t>Anti-Brp</t> antibody was used to label neuropil. (d) Example side view images of flies during calcium imaging experiments. Contact epoch (shaded pink and blue boxes) is defined as time between the first video frame in which a foreleg of the subject fly contacts the stimulus fly and the last video frame that the subject is in contact with the stimulus. (e) Example P1a activity upon contact with female (pink) or male (blue) abdomen in control (green) or fru mutant (purple) males. Shaded areas denote contact epochs. Median fluorescence intensity is used as baseline for calculation of ΔF/F for this panel. See Extended Data Fig. 5a for additional example flies. (f) P1a activity in contact epochs. Time is relative to the initiation of contact with stimulus fly. Data are sorted by duration of contact epochs. Fluorescence intensity observed during one second prior to the contact initiation is used as the baseline for ΔF/F0 calculation. See Extended Data Fig. 5b for unsorted data per fly. For control, 7 flies, 303 female epochs, 188 male epochs; for fru mutant, 7 flies, 283 female epochs, 235 male epochs. (g) Fraction of contact epochs in which P1a neurons become activated. Activation epoch if mean z-scored activity through epoch is over 1.96. See Extended Data Fig. 5c for data with a different threshold. Statistics by Fisher’s exact test; *** p<0.001, * p<0.05, n.s. p>0.05. (h) Mean P1a response across all contact epochs. Each dot is a fly and represents averaged peak ΔF/F0 observed during each contact epoch for the fly. Statistics by two-sided Wilcoxon rank sum test between control and fru mutant and by two-sided Wilcoxon signed rank test between response to females vs males. ** p<0.01.
632496 Mab Anti Bruchpilot Dshb Nc82 Mab Anti β Galactosidase Promega Z3781 Mab Anti Calcineurin β Subunit, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Vector Laboratories dapi vector laboratories h 1800 cholera toxin subunit b recombinant
(a) Representative confocal slice images of P1a neuron cell bodies in control vs fru mutant males in different social conditions. Flies were reared singly for 3 days upon eclosion and then paired with indicated partners for an additional 3-day period. (b) NLI of CRTC::GFP signal in P1a neurons of control vs fru mutant males in different conditions. Each dot represents mean NLI across cells per fly. Boxplot; median (horizontal line), first and third quartiles (box), and data within 1.5x IQR (whiskers). Statistics by Kruskal-Wallis test followed by a post hoc Tukey’s HSD test; *** p<0.001, ** p<0.01, * p<0.05, n.s. p>0.05. (c) Confocal images of P1a neurons expressing GCaMP in control and fru mutant males. Dotted area represents approximate plane for calcium imaging. <t>Anti-Brp</t> antibody was used to label neuropil. (d) Example side view images of flies during calcium imaging experiments. Contact epoch (shaded pink and blue boxes) is defined as time between the first video frame in which a foreleg of the subject fly contacts the stimulus fly and the last video frame that the subject is in contact with the stimulus. (e) Example P1a activity upon contact with female (pink) or male (blue) abdomen in control (green) or fru mutant (purple) males. Shaded areas denote contact epochs. Median fluorescence intensity is used as baseline for calculation of ΔF/F for this panel. See Extended Data Fig. 5a for additional example flies. (f) P1a activity in contact epochs. Time is relative to the initiation of contact with stimulus fly. Data are sorted by duration of contact epochs. Fluorescence intensity observed during one second prior to the contact initiation is used as the baseline for ΔF/F0 calculation. See Extended Data Fig. 5b for unsorted data per fly. For control, 7 flies, 303 female epochs, 188 male epochs; for fru mutant, 7 flies, 283 female epochs, 235 male epochs. (g) Fraction of contact epochs in which P1a neurons become activated. Activation epoch if mean z-scored activity through epoch is over 1.96. See Extended Data Fig. 5c for data with a different threshold. Statistics by Fisher’s exact test; *** p<0.001, * p<0.05, n.s. p>0.05. (h) Mean P1a response across all contact epochs. Each dot is a fly and represents averaged peak ΔF/F0 observed during each contact epoch for the fly. Statistics by two-sided Wilcoxon rank sum test between control and fru mutant and by two-sided Wilcoxon signed rank test between response to females vs males. ** p<0.01.
Dapi Vector Laboratories H 1800 Cholera Toxin Subunit B Recombinant, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Rockland Immunochemicals rabbit anti-red fluorescent protein
(a) Representative confocal slice images of P1a neuron cell bodies in control vs fru mutant males in different social conditions. Flies were reared singly for 3 days upon eclosion and then paired with indicated partners for an additional 3-day period. (b) NLI of CRTC::GFP signal in P1a neurons of control vs fru mutant males in different conditions. Each dot represents mean NLI across cells per fly. Boxplot; median (horizontal line), first and third quartiles (box), and data within 1.5x IQR (whiskers). Statistics by Kruskal-Wallis test followed by a post hoc Tukey’s HSD test; *** p<0.001, ** p<0.01, * p<0.05, n.s. p>0.05. (c) Confocal images of P1a neurons expressing GCaMP in control and fru mutant males. Dotted area represents approximate plane for calcium imaging. <t>Anti-Brp</t> antibody was used to label neuropil. (d) Example side view images of flies during calcium imaging experiments. Contact epoch (shaded pink and blue boxes) is defined as time between the first video frame in which a foreleg of the subject fly contacts the stimulus fly and the last video frame that the subject is in contact with the stimulus. (e) Example P1a activity upon contact with female (pink) or male (blue) abdomen in control (green) or fru mutant (purple) males. Shaded areas denote contact epochs. Median fluorescence intensity is used as baseline for calculation of ΔF/F for this panel. See Extended Data Fig. 5a for additional example flies. (f) P1a activity in contact epochs. Time is relative to the initiation of contact with stimulus fly. Data are sorted by duration of contact epochs. Fluorescence intensity observed during one second prior to the contact initiation is used as the baseline for ΔF/F0 calculation. See Extended Data Fig. 5b for unsorted data per fly. For control, 7 flies, 303 female epochs, 188 male epochs; for fru mutant, 7 flies, 283 female epochs, 235 male epochs. (g) Fraction of contact epochs in which P1a neurons become activated. Activation epoch if mean z-scored activity through epoch is over 1.96. See Extended Data Fig. 5c for data with a different threshold. Statistics by Fisher’s exact test; *** p<0.001, * p<0.05, n.s. p>0.05. (h) Mean P1a response across all contact epochs. Each dot is a fly and represents averaged peak ΔF/F0 observed during each contact epoch for the fly. Statistics by two-sided Wilcoxon rank sum test between control and fru mutant and by two-sided Wilcoxon signed rank test between response to females vs males. ** p<0.01.
Rabbit Anti Red Fluorescent Protein, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories ba 1000 goat anti mouse igg h l
(a) Representative confocal slice images of P1a neuron cell bodies in control vs fru mutant males in different social conditions. Flies were reared singly for 3 days upon eclosion and then paired with indicated partners for an additional 3-day period. (b) NLI of CRTC::GFP signal in P1a neurons of control vs fru mutant males in different conditions. Each dot represents mean NLI across cells per fly. Boxplot; median (horizontal line), first and third quartiles (box), and data within 1.5x IQR (whiskers). Statistics by Kruskal-Wallis test followed by a post hoc Tukey’s HSD test; *** p<0.001, ** p<0.01, * p<0.05, n.s. p>0.05. (c) Confocal images of P1a neurons expressing GCaMP in control and fru mutant males. Dotted area represents approximate plane for calcium imaging. <t>Anti-Brp</t> antibody was used to label neuropil. (d) Example side view images of flies during calcium imaging experiments. Contact epoch (shaded pink and blue boxes) is defined as time between the first video frame in which a foreleg of the subject fly contacts the stimulus fly and the last video frame that the subject is in contact with the stimulus. (e) Example P1a activity upon contact with female (pink) or male (blue) abdomen in control (green) or fru mutant (purple) males. Shaded areas denote contact epochs. Median fluorescence intensity is used as baseline for calculation of ΔF/F for this panel. See Extended Data Fig. 5a for additional example flies. (f) P1a activity in contact epochs. Time is relative to the initiation of contact with stimulus fly. Data are sorted by duration of contact epochs. Fluorescence intensity observed during one second prior to the contact initiation is used as the baseline for ΔF/F0 calculation. See Extended Data Fig. 5b for unsorted data per fly. For control, 7 flies, 303 female epochs, 188 male epochs; for fru mutant, 7 flies, 283 female epochs, 235 male epochs. (g) Fraction of contact epochs in which P1a neurons become activated. Activation epoch if mean z-scored activity through epoch is over 1.96. See Extended Data Fig. 5c for data with a different threshold. Statistics by Fisher’s exact test; *** p<0.001, * p<0.05, n.s. p>0.05. (h) Mean P1a response across all contact epochs. Each dot is a fly and represents averaged peak ΔF/F0 observed during each contact epoch for the fly. Statistics by two-sided Wilcoxon rank sum test between control and fru mutant and by two-sided Wilcoxon signed rank test between response to females vs males. ** p<0.01.
Ba 1000 Goat Anti Mouse Igg H L, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(a) Representative confocal slice images of P1a neuron cell bodies in control vs fru mutant males in different social conditions. Flies were reared singly for 3 days upon eclosion and then paired with indicated partners for an additional 3-day period. (b) NLI of CRTC::GFP signal in P1a neurons of control vs fru mutant males in different conditions. Each dot represents mean NLI across cells per fly. Boxplot; median (horizontal line), first and third quartiles (box), and data within 1.5x IQR (whiskers). Statistics by Kruskal-Wallis test followed by a post hoc Tukey’s HSD test; *** p<0.001, ** p<0.01, * p<0.05, n.s. p>0.05. (c) Confocal images of P1a neurons expressing GCaMP in control and fru mutant males. Dotted area represents approximate plane for calcium imaging. Anti-Brp antibody was used to label neuropil. (d) Example side view images of flies during calcium imaging experiments. Contact epoch (shaded pink and blue boxes) is defined as time between the first video frame in which a foreleg of the subject fly contacts the stimulus fly and the last video frame that the subject is in contact with the stimulus. (e) Example P1a activity upon contact with female (pink) or male (blue) abdomen in control (green) or fru mutant (purple) males. Shaded areas denote contact epochs. Median fluorescence intensity is used as baseline for calculation of ΔF/F for this panel. See Extended Data Fig. 5a for additional example flies. (f) P1a activity in contact epochs. Time is relative to the initiation of contact with stimulus fly. Data are sorted by duration of contact epochs. Fluorescence intensity observed during one second prior to the contact initiation is used as the baseline for ΔF/F0 calculation. See Extended Data Fig. 5b for unsorted data per fly. For control, 7 flies, 303 female epochs, 188 male epochs; for fru mutant, 7 flies, 283 female epochs, 235 male epochs. (g) Fraction of contact epochs in which P1a neurons become activated. Activation epoch if mean z-scored activity through epoch is over 1.96. See Extended Data Fig. 5c for data with a different threshold. Statistics by Fisher’s exact test; *** p<0.001, * p<0.05, n.s. p>0.05. (h) Mean P1a response across all contact epochs. Each dot is a fly and represents averaged peak ΔF/F0 observed during each contact epoch for the fly. Statistics by two-sided Wilcoxon rank sum test between control and fru mutant and by two-sided Wilcoxon signed rank test between response to females vs males. ** p<0.01.

Journal: Nature neuroscience

Article Title: A rapid and bidirectional reporter of neural activity reveals neural correlates of social behaviors in Drosophila

doi: 10.1038/s41593-023-01357-w

Figure Lengend Snippet: (a) Representative confocal slice images of P1a neuron cell bodies in control vs fru mutant males in different social conditions. Flies were reared singly for 3 days upon eclosion and then paired with indicated partners for an additional 3-day period. (b) NLI of CRTC::GFP signal in P1a neurons of control vs fru mutant males in different conditions. Each dot represents mean NLI across cells per fly. Boxplot; median (horizontal line), first and third quartiles (box), and data within 1.5x IQR (whiskers). Statistics by Kruskal-Wallis test followed by a post hoc Tukey’s HSD test; *** p<0.001, ** p<0.01, * p<0.05, n.s. p>0.05. (c) Confocal images of P1a neurons expressing GCaMP in control and fru mutant males. Dotted area represents approximate plane for calcium imaging. Anti-Brp antibody was used to label neuropil. (d) Example side view images of flies during calcium imaging experiments. Contact epoch (shaded pink and blue boxes) is defined as time between the first video frame in which a foreleg of the subject fly contacts the stimulus fly and the last video frame that the subject is in contact with the stimulus. (e) Example P1a activity upon contact with female (pink) or male (blue) abdomen in control (green) or fru mutant (purple) males. Shaded areas denote contact epochs. Median fluorescence intensity is used as baseline for calculation of ΔF/F for this panel. See Extended Data Fig. 5a for additional example flies. (f) P1a activity in contact epochs. Time is relative to the initiation of contact with stimulus fly. Data are sorted by duration of contact epochs. Fluorescence intensity observed during one second prior to the contact initiation is used as the baseline for ΔF/F0 calculation. See Extended Data Fig. 5b for unsorted data per fly. For control, 7 flies, 303 female epochs, 188 male epochs; for fru mutant, 7 flies, 283 female epochs, 235 male epochs. (g) Fraction of contact epochs in which P1a neurons become activated. Activation epoch if mean z-scored activity through epoch is over 1.96. See Extended Data Fig. 5c for data with a different threshold. Statistics by Fisher’s exact test; *** p<0.001, * p<0.05, n.s. p>0.05. (h) Mean P1a response across all contact epochs. Each dot is a fly and represents averaged peak ΔF/F0 observed during each contact epoch for the fly. Statistics by two-sided Wilcoxon rank sum test between control and fru mutant and by two-sided Wilcoxon signed rank test between response to females vs males. ** p<0.01.

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken-anti-GFP Aves Labs GFP-1020 Rabbit-anti-DsRed Clontech 632496 mAb anti-Bruchpilot DSHB nc82 mAb anti-β-Galactosidase Promega Z3781 mAb anti-Calcineurin (β-subunit) Sigma C0581 AlexaFluor488 goat-anti-chicken Life Technologies A11039 AlexaFluor568 goat-anti-rabbit Life Technologies A11036 AlexaFluor633 goat-anti-mouse Life Technologies A21052 Chemicals, peptides, and recombinant proteins Farnesol Sigma-Aldrich F203 Mineral Oil Fisher Scientific O122 VectaShield Vector Laboratories H-1000 SlowFade Gold Invitrogen S36938 SlowFade Diamond Invitrogen S36963 Experimental models: Organisms/strains D. melanogaster : MB247-DsRed, UAS-GCaMP6f 70 D. melanogaster : UAS-CRTC::GFP in attP40 This study D. melanogaster : UAS-CRTC::GFP in attP40 This study D. melanogaster : UAS-GFP in attP40 This study D. melanogaster : UAS-mCD8::mCherry (2nd Chr.)

Techniques: Mutagenesis, Expressing, Imaging, Activity Assay, Fluorescence, Activation Assay

REAGENTS AND RESOURCES

Journal: Nature neuroscience

Article Title: A rapid and bidirectional reporter of neural activity reveals neural correlates of social behaviors in Drosophila

doi: 10.1038/s41593-023-01357-w

Figure Lengend Snippet: REAGENTS AND RESOURCES

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken-anti-GFP Aves Labs GFP-1020 Rabbit-anti-DsRed Clontech 632496 mAb anti-Bruchpilot DSHB nc82 mAb anti-β-Galactosidase Promega Z3781 mAb anti-Calcineurin (β-subunit) Sigma C0581 AlexaFluor488 goat-anti-chicken Life Technologies A11039 AlexaFluor568 goat-anti-rabbit Life Technologies A11036 AlexaFluor633 goat-anti-mouse Life Technologies A21052 Chemicals, peptides, and recombinant proteins Farnesol Sigma-Aldrich F203 Mineral Oil Fisher Scientific O122 VectaShield Vector Laboratories H-1000 SlowFade Gold Invitrogen S36938 SlowFade Diamond Invitrogen S36963 Experimental models: Organisms/strains D. melanogaster : MB247-DsRed, UAS-GCaMP6f 70 D. melanogaster : UAS-CRTC::GFP in attP40 This study D. melanogaster : UAS-CRTC::GFP in attP40 This study D. melanogaster : UAS-GFP in attP40 This study D. melanogaster : UAS-mCD8::mCherry (2nd Chr.)

Techniques: Recombinant, Plasmid Preparation, Software

KEY RESOURCES TABLE

Journal: Neuron

Article Title: Thalamic regulation of sucrose-seeking during unexpected reward omission

doi: 10.1016/j.neuron.2017.03.036

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Each entry must be on a separate row; do not list multiple items in a single table cell. table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-cFos EMD Millipore ABE457 Secondary antibody Alexa Fluor 594 Life Technologies A20185 Bacterial and Virus Strains AAV5:CaMKIIα::hChR2(H134R)-eYFP University of North Carolina (UNC) vector core https://www.med.unc.edu/genetherapy/vectorcore N/A AAV5:CaMKIIα::eNpHR3.0-eYFP UNC vector core N/A AAV5:CaMKIIα::eYFP UNC vector core N/A Biological Samples Chemicals, Peptides, and Recombinant Proteins Fast Blue Polysciences, Inc 17740 Cholera Toxin Subunit B (Recombinant), Alexa Fluor 594 Conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"C34777","term_id":"2370918","term_text":"C34777"}} C34777 Muscimol BODIPY TMR-X conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"M23400","term_id":"340906","term_text":"M23400"}} M23400 Vectashield anti-fading mounting media Vector Laboratories H-1200 Critical Commercial Assays Blue diode-pumped solid state laser OptoEngine DPSS, 473 nm Yellow diode-pumped solid state laser OptoEngine DPSS, 593.5 nm Electrical Stimulator Grass Instruments S88X Shutter/coupler OzOptics HPUC-23-400/700-M-20AC-11-SH Infusion Machine Harvard Apparatus Model 11 plus Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains Male Sprague Dawley Rats Charles River N/A Oligonucleotides Recombinant DNA Software and Algorithms Cineplex Plexon, Inc N/A Offline Sorter Plexon, Inc N/A Neuroexplorer NEX Technologies N/A ANY-maze Stoelting Co N/A Graphic State Coulbourn Instruments N/A STATISTICA 6 Stat-Soft, Inc N/A Other Open in a separate window KEY RESOURCES TABLE

Techniques: Virus, Plasmid Preparation, Recombinant, Software

KEY RESOURCES TABLE

Journal: Neuron

Article Title: Thalamic regulation of sucrose-seeking during unexpected reward omission

doi: 10.1016/j.neuron.2017.03.036

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Each entry must be on a separate row; do not list multiple items in a single table cell. table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-cFos EMD Millipore ABE457 Secondary antibody Alexa Fluor 594 Life Technologies A20185 Bacterial and Virus Strains AAV5:CaMKIIα::hChR2(H134R)-eYFP University of North Carolina (UNC) vector core https://www.med.unc.edu/genetherapy/vectorcore N/A AAV5:CaMKIIα::eNpHR3.0-eYFP UNC vector core N/A AAV5:CaMKIIα::eYFP UNC vector core N/A Biological Samples Chemicals, Peptides, and Recombinant Proteins Fast Blue Polysciences, Inc 17740 Cholera Toxin Subunit B (Recombinant), Alexa Fluor 594 Conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"C34777","term_id":"2370918","term_text":"C34777"}} C34777 Muscimol BODIPY TMR-X conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"M23400","term_id":"340906","term_text":"M23400"}} M23400 Vectashield anti-fading mounting media Vector Laboratories H-1200 Critical Commercial Assays Blue diode-pumped solid state laser OptoEngine DPSS, 473 nm Yellow diode-pumped solid state laser OptoEngine DPSS, 593.5 nm Electrical Stimulator Grass Instruments S88X Shutter/coupler OzOptics HPUC-23-400/700-M-20AC-11-SH Infusion Machine Harvard Apparatus Model 11 plus Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains Male Sprague Dawley Rats Charles River N/A Oligonucleotides Recombinant DNA Software and Algorithms Cineplex Plexon, Inc N/A Offline Sorter Plexon, Inc N/A Neuroexplorer NEX Technologies N/A ANY-maze Stoelting Co N/A Graphic State Coulbourn Instruments N/A STATISTICA 6 Stat-Soft, Inc N/A Other Open in a separate window KEY RESOURCES TABLE

Techniques: Virus, Plasmid Preparation, Recombinant, Software

KEY RESOURCES TABLE

Journal: Neuron

Article Title: Thalamic regulation of sucrose-seeking during unexpected reward omission

doi: 10.1016/j.neuron.2017.03.036

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Each entry must be on a separate row; do not list multiple items in a single table cell. table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-cFos EMD Millipore ABE457 Secondary antibody Alexa Fluor 594 Life Technologies A20185 Bacterial and Virus Strains AAV5:CaMKIIα::hChR2(H134R)-eYFP University of North Carolina (UNC) vector core https://www.med.unc.edu/genetherapy/vectorcore N/A AAV5:CaMKIIα::eNpHR3.0-eYFP UNC vector core N/A AAV5:CaMKIIα::eYFP UNC vector core N/A Biological Samples Chemicals, Peptides, and Recombinant Proteins Fast Blue Polysciences, Inc 17740 Cholera Toxin Subunit B (Recombinant), Alexa Fluor 594 Conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"C34777","term_id":"2370918","term_text":"C34777"}} C34777 Muscimol BODIPY TMR-X conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"M23400","term_id":"340906","term_text":"M23400"}} M23400 Vectashield anti-fading mounting media Vector Laboratories H-1200 Critical Commercial Assays Blue diode-pumped solid state laser OptoEngine DPSS, 473 nm Yellow diode-pumped solid state laser OptoEngine DPSS, 593.5 nm Electrical Stimulator Grass Instruments S88X Shutter/coupler OzOptics HPUC-23-400/700-M-20AC-11-SH Infusion Machine Harvard Apparatus Model 11 plus Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains Male Sprague Dawley Rats Charles River N/A Oligonucleotides Recombinant DNA Software and Algorithms Cineplex Plexon, Inc N/A Offline Sorter Plexon, Inc N/A Neuroexplorer NEX Technologies N/A ANY-maze Stoelting Co N/A Graphic State Coulbourn Instruments N/A STATISTICA 6 Stat-Soft, Inc N/A Other Open in a separate window KEY RESOURCES TABLE

Techniques: Plasmid Preparation, Recombinant, Software

KEY RESOURCES TABLE

Journal: Neuron

Article Title: Thalamic regulation of sucrose-seeking during unexpected reward omission

doi: 10.1016/j.neuron.2017.03.036

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Each entry must be on a separate row; do not list multiple items in a single table cell. table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-cFos EMD Millipore ABE457 Secondary antibody Alexa Fluor 594 Life Technologies A20185 Bacterial and Virus Strains AAV5:CaMKIIα::hChR2(H134R)-eYFP University of North Carolina (UNC) vector core https://www.med.unc.edu/genetherapy/vectorcore N/A AAV5:CaMKIIα::eNpHR3.0-eYFP UNC vector core N/A AAV5:CaMKIIα::eYFP UNC vector core N/A Biological Samples Chemicals, Peptides, and Recombinant Proteins Fast Blue Polysciences, Inc 17740 Cholera Toxin Subunit B (Recombinant), Alexa Fluor 594 Conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"C34777","term_id":"2370918","term_text":"C34777"}} C34777 Muscimol BODIPY TMR-X conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"M23400","term_id":"340906","term_text":"M23400"}} M23400 Vectashield anti-fading mounting media Vector Laboratories H-1200 Critical Commercial Assays Blue diode-pumped solid state laser OptoEngine DPSS, 473 nm Yellow diode-pumped solid state laser OptoEngine DPSS, 593.5 nm Electrical Stimulator Grass Instruments S88X Shutter/coupler OzOptics HPUC-23-400/700-M-20AC-11-SH Infusion Machine Harvard Apparatus Model 11 plus Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains Male Sprague Dawley Rats Charles River N/A Oligonucleotides Recombinant DNA Software and Algorithms Cineplex Plexon, Inc N/A Offline Sorter Plexon, Inc N/A Neuroexplorer NEX Technologies N/A ANY-maze Stoelting Co N/A Graphic State Coulbourn Instruments N/A STATISTICA 6 Stat-Soft, Inc N/A Other Open in a separate window KEY RESOURCES TABLE

Techniques: Virus, Plasmid Preparation, Recombinant, Software