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Developmental Studies Hybridoma Bank
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Vector Laboratories
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Rockland Immunochemicals
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Vector Laboratories
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Millipore
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Image Search Results
Journal: Nature neuroscience
Article Title: A rapid and bidirectional reporter of neural activity reveals neural correlates of social behaviors in Drosophila
doi: 10.1038/s41593-023-01357-w
Figure Lengend Snippet: (a) Representative confocal slice images of P1a neuron cell bodies in control vs fru mutant males in different social conditions. Flies were reared singly for 3 days upon eclosion and then paired with indicated partners for an additional 3-day period. (b) NLI of CRTC::GFP signal in P1a neurons of control vs fru mutant males in different conditions. Each dot represents mean NLI across cells per fly. Boxplot; median (horizontal line), first and third quartiles (box), and data within 1.5x IQR (whiskers). Statistics by Kruskal-Wallis test followed by a post hoc Tukey’s HSD test; *** p<0.001, ** p<0.01, * p<0.05, n.s. p>0.05. (c) Confocal images of P1a neurons expressing GCaMP in control and fru mutant males. Dotted area represents approximate plane for calcium imaging. Anti-Brp antibody was used to label neuropil. (d) Example side view images of flies during calcium imaging experiments. Contact epoch (shaded pink and blue boxes) is defined as time between the first video frame in which a foreleg of the subject fly contacts the stimulus fly and the last video frame that the subject is in contact with the stimulus. (e) Example P1a activity upon contact with female (pink) or male (blue) abdomen in control (green) or fru mutant (purple) males. Shaded areas denote contact epochs. Median fluorescence intensity is used as baseline for calculation of ΔF/F for this panel. See Extended Data Fig. 5a for additional example flies. (f) P1a activity in contact epochs. Time is relative to the initiation of contact with stimulus fly. Data are sorted by duration of contact epochs. Fluorescence intensity observed during one second prior to the contact initiation is used as the baseline for ΔF/F0 calculation. See Extended Data Fig. 5b for unsorted data per fly. For control, 7 flies, 303 female epochs, 188 male epochs; for fru mutant, 7 flies, 283 female epochs, 235 male epochs. (g) Fraction of contact epochs in which P1a neurons become activated. Activation epoch if mean z-scored activity through epoch is over 1.96. See Extended Data Fig. 5c for data with a different threshold. Statistics by Fisher’s exact test; *** p<0.001, * p<0.05, n.s. p>0.05. (h) Mean P1a response across all contact epochs. Each dot is a fly and represents averaged peak ΔF/F0 observed during each contact epoch for the fly. Statistics by two-sided Wilcoxon rank sum test between control and fru mutant and by two-sided Wilcoxon signed rank test between response to females vs males. ** p<0.01.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken-anti-GFP Aves Labs GFP-1020 Rabbit-anti-DsRed Clontech
Techniques: Mutagenesis, Expressing, Imaging, Activity Assay, Fluorescence, Activation Assay
Journal: Nature neuroscience
Article Title: A rapid and bidirectional reporter of neural activity reveals neural correlates of social behaviors in Drosophila
doi: 10.1038/s41593-023-01357-w
Figure Lengend Snippet: REAGENTS AND RESOURCES
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken-anti-GFP Aves Labs GFP-1020 Rabbit-anti-DsRed Clontech
Techniques: Recombinant, Plasmid Preparation, Software
Journal: Neuron
Article Title: Thalamic regulation of sucrose-seeking during unexpected reward omission
doi: 10.1016/j.neuron.2017.03.036
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Each entry must be on a separate row; do not list multiple items in a single table cell. table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-cFos EMD Millipore ABE457 Secondary antibody Alexa Fluor 594 Life Technologies A20185 Bacterial and Virus Strains AAV5:CaMKIIα::hChR2(H134R)-eYFP University of North Carolina (UNC) vector core https://www.med.unc.edu/genetherapy/vectorcore N/A AAV5:CaMKIIα::eNpHR3.0-eYFP UNC vector core N/A AAV5:CaMKIIα::eYFP UNC vector core N/A Biological Samples Chemicals, Peptides, and Recombinant Proteins Fast Blue Polysciences, Inc 17740 Cholera Toxin Subunit B (Recombinant), Alexa Fluor 594 Conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"C34777","term_id":"2370918","term_text":"C34777"}} C34777 Muscimol BODIPY TMR-X conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"M23400","term_id":"340906","term_text":"M23400"}} M23400 Vectashield anti-fading mounting media Vector Laboratories H-1200 Critical Commercial Assays Blue diode-pumped solid state laser OptoEngine DPSS, 473 nm Yellow diode-pumped solid state laser OptoEngine DPSS, 593.5 nm Electrical Stimulator Grass Instruments S88X Shutter/coupler OzOptics HPUC-23-400/700-M-20AC-11-SH Infusion Machine Harvard Apparatus Model 11 plus Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains Male Sprague Dawley Rats Charles River N/A Oligonucleotides Recombinant DNA Software and
Techniques: Virus, Plasmid Preparation, Recombinant, Software
Journal: Neuron
Article Title: Thalamic regulation of sucrose-seeking during unexpected reward omission
doi: 10.1016/j.neuron.2017.03.036
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Each entry must be on a separate row; do not list multiple items in a single table cell. table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-cFos EMD Millipore ABE457 Secondary antibody Alexa Fluor 594 Life Technologies A20185 Bacterial and Virus Strains AAV5:CaMKIIα::hChR2(H134R)-eYFP University of North Carolina (UNC) vector core https://www.med.unc.edu/genetherapy/vectorcore N/A AAV5:CaMKIIα::eNpHR3.0-eYFP UNC vector core N/A AAV5:CaMKIIα::eYFP UNC vector core N/A Biological Samples Chemicals, Peptides, and Recombinant Proteins Fast Blue Polysciences, Inc 17740 Cholera Toxin Subunit B (Recombinant), Alexa Fluor 594 Conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"C34777","term_id":"2370918","term_text":"C34777"}} C34777 Muscimol BODIPY TMR-X conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"M23400","term_id":"340906","term_text":"M23400"}} M23400 Vectashield anti-fading mounting media Vector Laboratories H-1200 Critical Commercial Assays Blue diode-pumped solid state laser OptoEngine DPSS, 473 nm Yellow diode-pumped solid state laser OptoEngine DPSS, 593.5 nm Electrical Stimulator Grass Instruments S88X Shutter/coupler OzOptics HPUC-23-400/700-M-20AC-11-SH Infusion Machine Harvard Apparatus Model 11 plus Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains Male Sprague Dawley Rats Charles River N/A Oligonucleotides Recombinant DNA Software and Algorithms Cineplex Plexon,
Techniques: Virus, Plasmid Preparation, Recombinant, Software
Journal: Neuron
Article Title: Thalamic regulation of sucrose-seeking during unexpected reward omission
doi: 10.1016/j.neuron.2017.03.036
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Each entry must be on a separate row; do not list multiple items in a single table cell. table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-cFos EMD Millipore ABE457 Secondary antibody Alexa Fluor 594 Life Technologies A20185 Bacterial and Virus Strains AAV5:CaMKIIα::hChR2(H134R)-eYFP University of North Carolina (UNC) vector core https://www.med.unc.edu/genetherapy/vectorcore N/A AAV5:CaMKIIα::eNpHR3.0-eYFP UNC vector core N/A AAV5:CaMKIIα::eYFP UNC vector core N/A Biological Samples Chemicals, Peptides, and Recombinant Proteins Fast Blue Polysciences, Inc 17740 Cholera Toxin Subunit B (Recombinant), Alexa Fluor 594 Conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"C34777","term_id":"2370918","term_text":"C34777"}} C34777 Muscimol BODIPY TMR-X conjugated ThermoFisher Scientific {"type":"entrez-nucleotide","attrs":{"text":"M23400","term_id":"340906","term_text":"M23400"}} M23400 Vectashield anti-fading mounting media Vector Laboratories H-1200 Critical Commercial Assays Blue diode-pumped solid state laser OptoEngine DPSS, 473 nm Yellow diode-pumped solid state laser OptoEngine DPSS, 593.5 nm Electrical Stimulator Grass Instruments S88X Shutter/coupler OzOptics HPUC-23-400/700-M-20AC-11-SH Infusion Machine Harvard Apparatus Model 11 plus Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains Male Sprague Dawley Rats Charles River N/A Oligonucleotides Recombinant DNA Software and Algorithms Cineplex Plexon, Inc N/A Offline Sorter Plexon, Inc N/A Neuroexplorer NEX Technologies N/A
Techniques: Plasmid Preparation, Recombinant, Software
Journal: Neuron
Article Title: Thalamic regulation of sucrose-seeking during unexpected reward omission
doi: 10.1016/j.neuron.2017.03.036
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Each entry must be on a separate row; do not list multiple items in a single table cell. table ft1 table-wrap mode="anchored" t5 REAGENT or
Techniques: Virus, Plasmid Preparation, Recombinant, Software